Slashdot | How To Check Yourself For Abnormal Genes

Slashdot | How To Check Yourself For Abnormal Genes: "I have to disagree with you here, at least for checking a specific, limited set of loci. IAAMB (molecular biologist) but I don't have 'massive' experience with PCR and yet I've never had trouble getting it to work by following standard protocols using quality reagents (e.g. from NEB [neb.com]) and primers (from IDT [idtdna.com]). As long as the DIY guide included directions to use IDT's software to assist them in choosing primers and to determine the annealing temperature to use during the PCR cycle, I don't see why your typical DIYer with access to some old lab equipment wouldn't be able to get it to work as long as the DNA prep was good."